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After 24 h a volume equivalent to OD 600 = 0.2/mL was harvested and centrifuged at 9 C

Purity, concentration, and integrity of RNA was assessed by NanoDrop Spectrophotometer (Thermo Fisher Scientific) and by Eukaryote Total RNA Nano chip analysis on a Bioanalyzer (Agilent Technologies) at the Stanford Protein and Nucleic Acid Facility

297 The specific mechanisms include the activation of the TLR4/NF-B pathway, which upregulates M1 phenotypic markers (such as TNF- and CD86) while inhibiting the expression of M2 phenotypic markers (such as Arg-1 and CD206)

Altogether, the increase in the amounts observed for acetate, glycerophosphocholine and uridine nucleotides in the presence of the TNAP inhibitor suggests reduction in lipogenesis that could reflect the reduction in the number of neurites per cell

31 CallaghanR

Consistently, independent isotopic labelling studies in human lung cancer indicated minimum glutamine utilization in vivo, with glucose and lactate being the main contributors to the TCA cycle 9,45