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Ghrelin and ibutamoren recognition In our structure, the N-terminal part of ghrelin adopts an extended conformation and inserts into a deep pocket of GHSR (Fig

Then, the cells were washed and suspended in Roswell Park Memorial Institute-1640 medium (RPMI-1640, Sigma-Aldrich) supplemented with 2 g/L sodium bicarbonate, 10% fetal bovine serum (FBS, Sigma-Aldrich), 2% glutamine, and 100 U/mL penicillin-0.1 mg/mL streptomycin (Sigma-Aldrich) and incubated in vitro with RPMI medium (control), tert -Butyl hydroperoxide (tBHP: 200 M) or tBHP plus Ab (22.5 mg/mL) for 30 min at 37C

Reactive oxygen species control protein degradation at the mitochondrial import gate

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A plot with 4 dimensions is virtually impossible to display graphically, and equally difficult to analyse