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Generation of Enteroid Monolayers Duodenal biopsies were obtained from five healthy individuals, two pediatrics (ages 2 and 5 years) and three adults (ages 25, 27, and 81 years) through endoscopy or surgical procedure

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The cells were subsequently washed with PBS and analyzed via flow cytometry (LSRFortessa

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injections of 100 nmol kg 1 MK-801 ( n = 8 rats), 100 nmol kg 1 GLP-1 ( n = 8 rats), 100 nmol kg 1 GLP-1MK-801 ( n = 8 rats) or vehicle (isotonic saline, n = 8 rats) for three consecutive days while being offered the choice between chow and kaolin