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In HT-29 cells, luteolin downregulates anti-apoptotic proteins and induces cell cycle arrest ( 3.3 Modulation of amino acid metabolism Amino acid metabolism provides both metabolic substrates and regulatory signals critical for tumor growth

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Twenty-four hours later, cells were lifted, washed with cold PBS, and stained with PE-Annexin V antibody and 7-AAD

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The highly efficient reaction, as demonstrated by very short reaction times reported by Taylor et al., allows for complete reaction with the relevant biological targets