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Finally, it is crucial to explore the key enzymes involved in CAT production by gut microbiota metabolism and their regulatory factors, evaluate the therapeutic potential of engineered probiotics (e.g., CAT-synthesizing strains) or exogenous CAT delivery systems (e.g., cartilage-targeted nanocarriers) for OA, and provide multi-level evidence for CAT-targeted OA therapy based on the gut-joint axis

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Phenotypic traits (morphological and physiological traits) are commonly used to estimate genetic diversity because they are easy to measure

Damaged proteins are eliminated by enzymes known as metalloproteinases

Functional Evaluation: Quantitative motion tests (range, stability, strength) and pain scales