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The addition of exogenous ROS results in resensitization of cells with high VRK2 expression to GSH inhibition, which is consistent with the fact that PC cells with low expression of VRK2 are susceptible to GSH inhibition attributes to its high basal ROS level, suggesting that cells in a state of oxidative stress have enhanced dependence on the GSH system

This resin works well with small and large proteins tagged with GST in batch or column purifications (Low Flow)

doi: 10.3390/ijms242216042 23 WangYMouYKWangHRSongXYWeiSZRenCet al

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A ThermoMax M5 microplate reader was used to measure the absorbance at 450 nm

Grx1-roGFP2 emits light at 500530 nm upon stimulation with either 405 nm or 488 nm wavelength