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In T2DM, upregulation of AMPK activity has been shown to inhibit mTOR, reducing autophagic vacuoles and potentially improving cell function

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After blocking, membranes were incubated with primary antibodies against SLC7a11 (1:1000, Abcam), GPX4 (1:1,000, Abcam), and PPAR (1:1,000

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