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According to the method disclosed by the invention, the crude semaglutide is prepared by carrying out liquid-phase one-step reaction on two segments of fragment peptides in a liquid phase, in the final liquid chromatography purification step, impurities are not defective peptides lacking one or more amino acids, but are uncondensed partial fragments, the purification difficulty is not caused, in addition, the uncondensed partial peptide fragments can be recycled, and the cost is saved

Rohatgi PR, Swisher SG, Correa AM, Wu TT, Liao Z, Komaki R, Walsh G, Vaporciyan A, Lynch PM, Rice DC , Roth JA, Ajani JA

In case of the transporter-mediated prodrug approach, the rational design of prodrugs should take into account the functional expression and structural requirements for substrate binding and translocation via the transporter, as well as capability to be bioconverted by an enzyme at the target site (74)

Epitalon has been found to affect enzymatic functions [91], including those of the digestive system [92,93]

Rohatgi PR, Swisher SG, Correa AM, Wu TT, Liao Z, Walsh GL, Vaporciyan AA, Rice DC , Fukami N, Roth JA, Ajani JA

Mechanisms of hepatic stellate cell activation