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DOI: 10.3390/biomedicines8100390

The excretion of intact alcohol through sweat and transdermal diffusion provides another source to estimate BAC using transdermal alcohol concentration (TAC)

The reaction was performed in a mixture containing 1.85 mL of 50 mM potassium phosphate buffer (pH 7.0), 0.5 mL of 0.5 mM ascorbate (AsA), 0.5 mL of 0.1 mM H 2 O 2 , and 0.15 mL of the enzyme extract