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The culture conditions for each strain were as follows: Staphylococcus hyicus , Streptococcus suis , Escherichia coli , and Pasteurella multocida were cultured on blood agar containing 5% sheep blood at 37 C for 1618 h

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In addition to its role in GSH biosynthesis, the -glutamyl cycle also contributes to the degradation of extracellular glutathione and amino acid recycling through the action of membrane-associated -glutamyl transferase (GGT)