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A CFX384 Touch Real-Time PCR Detection instrument (BioRad) was used, and the thermocycler conditions included a pre-incubation at 95 C (10 min) and 40 cycles of 95 C (15 s), 5560 C (30 s), 72 C (30 s), and a final elongation 72 C (4 min)

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Like basal-like tumours, it is triple negative (ER-, PR-, HER2-) but differs in having low Ki67 expression and elevated markers associated with epithelial-mesenchymal transition, indicating a distinct biological behaviour (142, 143)

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A cold-adapted tyrosinase with an abnormally high monophenolase/diphenolase activity ratio originating from the marine archaeon Candidatus Nitrosopumilus koreensis