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Originally identified in a screen for compounds selectively lethal to RAS-mutant cancer cells, RSL3 triggers ferroptotic cell death by inactivating GPX4, leading to the accumulation of toxic amounts of reactive oxygen species (ROS) and subsequent oxidative membrane damage [1]

On the other hand, icaritin promotes mitochondrial autophagy and increases autophagy flux, inducing cell death in cancer cells

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[2] Structure [edit] In bacteria, acylating acetaldehyde dehydrogenase forms a bifunctional heterodimer with metal-dependent 4-hydroxy-2-ketovalerate aldolase

VEGF suppresses T-lymphocyte infiltration in the tumor microenvironment through inhibition of NF-B-induced endothelial activation

The intracellular iron level was also assessed by using FerroOrange (F374-10, Dojindo, Japan)