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Western blotting HTMCs were grown in the above-mentioned TMC medium in five 12-well plates at a density of 5 10 4 /ml for 24 h, and the medium was replaced with a new one, containing 1 l of water (negative control), TGF-1 (20 g/ml) or glucaric acid (10 mM) for 10 min

Eur J Pharmacol 379:1931 Jelovac N, Sikiric P, Rucman R et al (1999b) The effect of a novel pentadecapeptide BPC 157 on development of tolerance and physical dependence following repeated administration of diazepam