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10.1002/ptr.6169 42 HoughtonP

Selank alone did not significantly alter gene expression in IMR-32 cells

Nat Rev Gastroenterol hepatol

Correlation analysis was conducted for the expression levels of tested microRNAs and the activities of glutathione reductase (GR), total superoxide dismutase (SOD), catalase (CAT), concentration of serum thiol groups and the level of Nrf2 mRNA

The target sequences were amplified using the following primers: Library preparation and sequencing Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs Inc., UK), and mRNA was isolated using a Poly(A) RNA Selection Kit (Lexogen, Inc., Austria)