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Tryptone, yeast extract, Terrific Broth (TB), agar, isopropyl--d-1-thiogalactopyranoside (IPTG) was purchased from Lab Empire

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b , Left, size-exclusion chromatography profile of purified SLC33A1 showing UV absorbance at 280 nm

In addition, suppression of GSTA2 expression in the metastatic HCC cells further intensified the effect of H 2 O 2 , leading to a significantly higher intracellular ROS level and lower proliferation rate (Fig

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Unless otherwise indicated, BMDMs or iBMDMs were routinely plated at 4 10 5 cells/well in 24-well tissue culture plates (BD Falcon) or 1 10 5 cells/well in 96-well flat bottom tissue culture plates