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The two histidines H626/628 shown to be critical for copper binding and thus catalytic activity 39,40 were mutated to glutamine with the QuikChange site-directed mutagenesis kit using forward primer: ATC TGG CAC GAC TGT CAA AGG CAA TAC CAC AGC ATG and reverse primer: CAT GCT GTG GTA TTG CCT TTG ACA GTC GTG CCA GAT

Gewandter JS, Culakova E, Davis JN, Gada U, Guido JJ, Bearden JD,

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[17] [18] In patients with persistent symptoms despite treatments for coeliac disease, IgE-mediated allergic diseases, including food allergy, allergic rhinitis, and asthma, should be considered in the differential diagnosis, as they may coexist with celiac disease