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After 24 h, fresh cell media with or without 5FU was applied to the cells and the cells were treated for a further 72 h

doi: 10.1016/j.cell.2016.12.034, 95 MuraoA.AzizM.WangH.BrennerM.WangP

To identify the specific roles of Gstp1 and 2 in neurite formation, we adopted the gain-of-function approach by overexpressing Gstp1 or 2 in primary cortical neurons (Figure 6)