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After 24 h of incubation, the cells were harvested, washed with PBS, and stained with an anti-CD86-APC antibody (1:100 dilution) at 4 C for 30 min

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J Pharmacol Exp Ther 282(3):15331540 Thompson RA, Isin EM, Li Y, Weidolf L, Page K, Wilson I, Swallow S, Middleton B, Stahl S, Foster AJ, Dolgos H, Weaver R, Kenna JG (2012) In vitro approach to assess the potential for risk of idiosyncratic adverse reactions caused by candidate drugs

We know that within five to ten minutes of treatment with GmPcide, the membranes start to become permeable and allow things that normally should be excluded to enter into the bacteria, which suggests that those membranes have been damaged. This can disrupt the bacterias own functions, including those that cause damage to their host, and make the bacteria less effective at combating the hosts immune response to infections