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Measurement of hair density and total hair count All subjects placed their head into a hair photograph device (Canfield Scientific), and photographs were taken at fixed distance, angle and lighting with an EOS Rebel T6i digital camera (Canon)

doi: 10.3389/fimmu.2025.1477437 Received 07 August 2024 Accepted 20 January 2025 Published 10 February 2025 Volume 16 - 2025 Edited by Nejat Dalay, Istanbul University, Trkiye Reviewed by Junwei Luo, Henan Polytechnic University, China Mario Perez-Medina, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico Updates Copyright 2025 Hu, Tian, Liu, Hou, wu, Wang and Shu

Interestingly, a case study of a 60-year-old man who had been regularly consuming 3 quarts of soy milk daily found that his estradiol and estrone levels were 4 times higher than the clinical upper-limit reference values for men [2]

Western blotting Loading was performed at 40 g mass and proteins were separated by SDS-PAGE polyacrylamide gel electrophoresis

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