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Adv Ther 42 , 12511264 (2025)

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Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)

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This makes it particularly valuable for comparative studies examining synergistic hormone regulation and for research requiring robust, physiologically-appropriate GH elevation