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Substrate concentrations were as follow: (i) 5 mM pyruvate (Sigma Aldrich, Cat # P76225) + 5 mM malate (Sigma Aldrich, Cat # M7397) + 4 mM ADP (Sigma Aldrich, Cat # A5285) (PMA), (ii) 5 mM succinate (Sigma Aldrich, Cat # S9512) + 2 M rotenone (Sigma Aldrich, Cat # R8875) + 4 mM ADP (SRA) or (iii) 2 mM malate + 4 mM ADP (MA)

You can keep the same reconstitution ratio and simply draw a different volume

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Colorectal Cancer Mortality in Relation to Glucose - 6 - Phosphate Dehydrogenase Deficiency and Consanguinity in Sardinia: A Spatial Correlation Analysis

Your skin may start to sag , making you look older than you actually are