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The experimental program of transaldolase and Hif1a: Through with methanol incubation 30 minutes, then with 2 times (each 10 minutes) of TBS-T washing, use the ProBloot membrane strip buffer subsequently in 50 of following incubations 30 minutes, peel off above-mentioned trace with 100ml or more TBS-T washing 2 times (each 30 minutes) then.The scanning trace is to check out fully stripped in laser scanner.With methanol activation trace 5 seconds, with water washing 5 minutes, then with TBST washing 15 minutes.At room temperature with the closed reagent among 5% TBS-T sealing trace 1 hour, subsequently with TBS-T washing 3 times (1 time, about 15 minutes

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And after sitting through those lectures and Mari enlightening us on all of her research and what she's seen, I left there with Ian and he looked at me and was like this