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Inclusion criteria Written informed consent was obtained from all 50 patients after providing information about the nature, content, and scope of the study

4.1 Enzymatic mechanisms 4.1.1 The glutathione (GSH)-GPX4 axis Ferroptosis is characterized by the hallmark feature of intracellular lipid peroxide accumulation, with the glutathione-glutathione peroxidase 4 (GSH-GPX4) axis playing a pivotal role in suppressing lipid peroxidation

Equivalent amounts of RNA (500 ng) was used in each reaction and 5X VILO reaction mix and10 SuperScript enzyme mix added and samples reacted at 42 C for 1 h