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In a healthy male body, LH is released by the pituitary gland and signals the testes to produce testosterone and sperm

Then, the mixture was incubated at 30 C for 10 min

10.1016/j.drudis.2010.07.005 39

The most common forms of ROS are O 2 - , hydroxyl radical (OH - ), hydrogen peroxide (H 2 O 2 ), and peroxynitrite (ONOO - ) (67)

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In the next step, the oxidation status was estimated by the GSH detection test (GSH-Glo Gluthation assay, Promega, Germany) which provides a simple and sensitive luminescent method