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Coimmunoprecipitation (co-IP) assay and immunoblotting Striatum tissues and cells were lysed using RIPA lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, and 0.5% NP-40) containing protease and phosphatase inhibitors for 1 h on ice and sonicated 3 times using a Sonics VC750 ultrasonic processor

[DOI] [PMC free article] [PubMed] [Google Scholar] 178.Frassinetti S., Moccia E., Caltavuturo L., Gabriele M., Longo V., Bellani L., Giorgi G., Giorgetti L

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