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I now believe that the gait abnormality was caused by pantothenic acid inhibiting the transport of biotin from my food, making a marginal deficiency into a severe deficiency, and that adding the biotin at night completely abolished this effect

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However, affinity to NADH is substantially weakened compared to Peredox ( K d(NADH) 1.2) [23] and for each NAPstar is one to two orders of magnitude lower than for NADPH ( Figure 1D,E )

We have developed the present protocol to avoid this methodological problem

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