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Continuous data was calculated using either the student's t -test or Mann Whitney U -test

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High-mobility group box 1 inhibitor Boxa alleviates neuroinflammation-induced retinal ganglion cell damage in traumatic optic neuropathy

The gut microbiota produces an array of bioactive metabolites, such as short-chain fatty acids (SCFAs), neurotransmitter precursors, and uremic toxins, that mediate communication between the gut, brain, and kidneys (Lin et al., 2025)

(4) Clinically, ephedrine is commonly used to treat anesthesia-induced hypotension, particularly during spinal, epidural, and general anesthesia

Cell synchronization and flow cytometry analysis Cells were synchronized at the G1/early S-phase boundary using a double-thymidine (DT) block protocol: cells were cultured with 2 mM thymidine for 16 h, then released to thymidine-free medium for 8 h, and with 2 mM thymidine for another 16 h