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TGP-free tt SI was then released from the column by His-tag-free 3C protease digestion, concentrated using a 100-kDa cutoff filtration membrane, heated at various temperatures, and used for FSEC-TS assay with the same setup as for GFP fusion protein except that the excitation and emission wavelength pair was changed to 280/350 nm

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Selective disruption of mitochondrial thiol redox state in cells and in vivo

In JRE #2461 with RFK Jr

Such upregulation most frequently occurs via loss-of-function mutations in KEAP1, the E3 ubiquitin ligase that degrades NRF2

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