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However, when administering a lower dose of 2APB, i.e., 1 mg/kg, the protective effect on APAP tolerance was observed only in wild-type mice, for which survival rate was 100% at 60 h post-2APB administration

The membranes were then incubated with HRP-labeled anti-rabbit, anti-mouse, or anti-goat (Proteintech) secondary antibodies

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