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Subsequently, the gel was submerged in a solution containing 0.5 N NaOH and 0.6 M NaCl for 15 min each to denature the DNA, followed by neutralization of the gel pH in a buffer containing 1.5 M NaCl and 0.5 M Tris-HCl (pH 7.5) for 30 min, and finally in 10X SSC (pH 7)

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doi:10.1158/1535-7163.885.3.7

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Endurance was evaluated by measuring the running time in seconds on the treadmill after 30 and after 60 days