glutathione sepharose 4b compatibility An alternative easy method for antibody purification and analysis of protein–protein interaction using GST fusion proteins immobilized onto glutathione–agarose | Analytical and Bioanalytical Chemistry transferase (GST) tag Transglutaminase 2 crosslinks the glutathione
Transglutaminase 2 crosslinks the glutathione S transferase tag, impeding proteinprotein interactions of the fused protein Experimental & Molecular Medicine Activated ThiolSepharose 4B lyophilized powder Sigma Aldrich CleaTag PreScission Protease Glutathione Sepharose 4B, Cytiva, affinity resin, pack of 10 mL Millipore Glutathione High Capacity Magnetic Agarose Beads, (1:1 suspension in a 30% ethanol solution) 1 ml Iron How to choose between affinity tags for protein purification Biotage
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