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in mouse models, stimulation of the kynurenine pathway was observed and the levels of several pathway metabolites including 3-hydroxykynurenine, quinolinic acid, and kynurenic acid are elevated (Notarangelo et al., 2014)

To get an estimate of the CAT activity, GR activity, and GPx activity, absorbance was measured at 570 nm, 340 nm, and 420 nm, respectively.[ , , ] Steroid hormones assay The serum levels of testosterone hormone (TH), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were analyzed utilizing rat-specific ELISA kits that were purchased from Qayee-Bio Life Science, China, and the assays were performed based on the competitive binding principle

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These biomarker clusters, in turn, formed 7 groups based on fold changes of free carnitine, selected acylcarnitines, the branched chain amino acids, lactate, and pyruvate and reveal patterns of metabolic dysregulation in autism

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