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Abbreviations O2PLS: Bidirectional orthogonal partial least squares PCA: Principal component analysis HCA: Hierarchical cluster analysis PCR: Polymerase chain reaction OTU: Operational taxonomic units GCMS: Gas chromatographymass spectrometry NMDS: Non-metric multidimensional scaling UPG MA: Unweighted Pair-group Method with Arithmetic Mean LEfSe: Linear discriminant analysis effect size LDA: Linear discriminant analysis Pi: Among-module connectivity Zi: Within-module connectivity Asp: Aspartic acid Pro: Proline Glu: Glutamic acid Thr: Threonine Phe: Phenylalanine Ser: Serine Ala: Alanine GABA: -Aminobutyric acid His: Histidine Trp: Tryptophan Lys: Lysine Val: Valine Arg: Arginine Gly: Glycine Cys: Cystine Leu: Leucine Iso: Isoleucine Tyr: Tyrosine Met: Methionine References Ali H, Paetzold R, Brueckner H (2006) Determination of L- and D-amino acids in smokeless tobacco products and tobacco

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