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For Atto594 labeling, Atto594-OSu ester (AttoTec) was solved in DMSO, and 2 l of this stock solution (7 mM) was incubated with 2 l of amino-modified ASO (6 g l 1 ) in 16 l of labeling buffer (20 parts PBS, pH 7.4, plus 1 part 0.2 M aqueous sodium bicarbonate, pH 9) at room temperature overnight

FBW7-NRA41-SCD1 axis synchronously regulates apoptosis and ferroptosis in pancreatic cancer cells

(2016) was based on the use of carbodiimide chemistry to conjugate a poly(N-vinylpyrrolidone) nanoscaffold to insulin: the obtained nanosystems were protected by protease degradation and capable to link to insulin receptors, trigger insulin signaling and promote neuroprotection effects against the dysfunction induced by amyloid-

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Additionally, whole tissue lysates were prepared from four discarded human steatotic livers deemed unsuitable for transplant