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Both protein and ligand structures were then taken to perform docking using AutoDock Vina 40 for zinc metalloproteins 41

Blood samples were collected by venipuncture in Vacutainer CPT Mononuclear Cell Preparation Tubes (BD, Franklin Lakes, NJ) with sodium heparin, immediately placed in ice water, and then centrifuged at 2,000 g at 4C for 10 min

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These include oral formulations such as pramipexole and ropinirole, the transdermal patch rotigotine, and subcutaneous apomorphine for acute "off"-period rescue [104]