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Subsequently, cells were fixed in cold 100% methanol for 8 minutes at 20C

You can use standard insulin syringes

There are a number of steps that go into any affinity purification protocol and each, too, must be carefully considered

Before ferroptosis was conceptualized, studies had already detected reduced GPX activity and GSH levels in the cerebrospinal fluid (CSF) of patients (Suzuki et al., 1983)