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Nevertheless, the discovery of an extra-hepatic IGF-I production in the following years[18, 45], made it necessary to spand the conception of the GH/IGF-I axis, being well accepted nowadays that pituitary GH is able to induce IGF-I synthesis in both liver (~75%) and other tissues[23, 46, 47], which subsequently, is able to act in an endocrine/paracrine/autocrine manner

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Cells were stimulated with 1.5 M oligomycin, 1 M carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), and 0.5 M antimycin A/rotenone

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