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Oocytes that failed to develop into the GVBD stage after 2 h were discarded, and the remaining GVBD stage oocytes were then continuously incubated to assess PBE development, indicated by the production of first polar body, in 14 h, 16 h and 20 h afterward

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When necessary, cerebrospinal fluid toxicology analysis and cerebrospinal fluid pressure measurement should be performed to enable the early detection of central nervous system lesions and the implementation of timely treatment plans

PMID 11665618