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Peptides were then separated over 100 m 15 cm ReproSil-Pur C18-AQ 1.5-m silica beads (Beijing Qinglian Biotech Co., Ltd, Beijing, China), at a flow rate of 300 nL/min using a gradient: 04 min (510% B), 446 min (1024% B), 4653 min (36% B), 5354 min (95% B), 5460 (95% B)