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While slower than at 37 C, metabolism at 4 C favors the intracellular acidification, in turn inhibiting pH-sensitive enzymes at rate-limiting steps of glycolysis, the Rapoport-Luebering shunt, and the pentose phosphate pathway (PPP) 11,12

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Supplementing it, particularly topically, aims to restore some of that youthful regenerative signaling

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2023 titled Further evidence of relationship between thiamine blood level and cognition in chronic alcohol-dependent adults: Prospective Pilot Study of an inpatient detoxification with oral supplementation protocol, improvements in cognition and thiamine blood levels have been observed over about 14 days of supplementation