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2) Neuroblastoma cells are infected with 50 HAU of H1N5, co-cultured with M1 antigen-specific hybridoma, measured by IL-2 secretion

After digestion and centrifugation, cells were washed twice with PBS and resuspended in a binding buffer

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Thiamin supplementation prevented any further increase in fasting glucose and insulin concentrations compared with placebo treatment but did not reduce the hyperglycemia (45)

Identifying at-risk individuals and treating them in a prodromal phase based on their driving pathophysiological process could prevent the manifestation of PD, or at least alter the disease progression (Ascherio and Schwarzschild, At present, two approaches could be feasible to investigate this matter: the a-priori stratification of study cohorts (based on assumed mitochondrial dysfunction) or the in-vivo assessment of mitochondrial dysfunction (by neuroimaging methods or blood biomarkers) (Prasuhn et al., 2019)