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PubMed PMID: 19240062

Cells were aliquoted and stored at 180 C until use

Any alterations in the signaling pathways that regulate fibroblast function can impact collagen production and, consequently, the resilience of the ACL

The inability of 5-cholesten-3-one to serve as substrate for the bacterial ketosteroid isomerase was noted in the original publication (Talalay and Wang, 1955), and the purified mammalian GST A3-3 from different sources also lack detectable activity with this steroid (unpublished work in our laboratory)