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Briefly, hiPSC colonies were detached with collagenase type IV, washed with DMEM:F12, and transferred to an uncoated polystyrene petri dish (VWR) in neural induction medium containing DMEM:F12 supplemented with 20% KnockOut Serum Replacement, 1X MEM-NEAAs, 2mM GlutaMax, penicillin (100 units/mL)/streptomycin (100 g/mL), 0.1mM 2-mercaptoethanol, 2 mM dorsomorphin and 2 mM A83-01