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For cortical organoid generation, iPSCs were cultured on mouse embryonic fibroblast feeder (MEF) cells in stem cell medium consisting of Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12 (DMEM:F12) supplemented with 20% KnockOut Serum Replacement, 1X MEM-non-essential amino acids (NEAA), 1X GlutaMAX, 1X penicillin-streptomycin, 1X 2-mercaptoethanol, and 10 ng/mL bFGF in a 5% CO 2 , 37C, 90% relative humidity incubator as previously described with minor modifications (Qian et al., 2018) (see Supplementary Table 1 for catalog numbers for all reagents)

doi:10.1073/pnas.1607571113 Nicolson GL, Rosenblatt S, de Mattos GF, et al

Increased Risk of Headaches Some studies have suggested that taking supplements may increase the risk of headaches

Melatonin ameliorates cognitive deficits through improving mitophagy in a mouse model of Alzheimers disease

17 (Suppl 2): 420444