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Rodriguez Padial L, Baron-Esquivias G, Hernandez Madrid A, Marzal Martin D, Pallares-Carratala V, de la Sierra A (2016) Clinical experience with diltiazem in the treatment of cardiovascular diseases

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The melting profile of the sample (2.5 M) was monitored at 228, 220, 225, and 218 nm for Ec GUS, Ee GUS, Lr GUS, and Bd GUS, respectively, from 20 C to 94 C

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For the assembly of the DDA1-DET1-DDB1-Ube2e2-COP1-c-Jun complex, untagged DDB1, TSF-tagged human DET1, N-terminal GST- and C-terminal His-tagged COP1, untagged DDA1, MBP-tagged Ube2e2 S76G-C139S and MBP-tagged c-Jun were co-transfected into Expi293F cells as previously described

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