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This result is surprising because H 2 O 2 treatment creates an oxidative environment, whereas a decrease in TPI activity shifts the NADPH/NADP+ ratio towards a reductive environment (Ralser et al

The results indicated that when the chromatography was performed with a pH 5.0 acetate buffer at a flow rate of 1 mL min 1 , GSH was purified from fermentation broth with the yield of 91.22% and purity of 60.03%