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Colon tumour cell death causes mTOR dependence by paracrine P2X4 stimulation

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Q., Jiang, J

Heart tissue samples were weighed, homogenized with 8 times of 2.5 mM ammonium acetate in methanol, and 20 L of the extraction from each sample was used for the assay

Figure 2 NF1, PTEN, and TSC1/TSC2 act as negative regulators of mTOR complex 1 (mTORC1), of which single gene mutations lead to enhanced mTOR activity in the brain in various mouse models (60, 62, 65)