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Louis, MO), and ionomycin (1 g/ml) (Sigma-Aldrich), then permeabilized and fixed with Cytofix/Cytoperm kit (BD Biosciences) and stained for IFN- and IL-17A following the manufacturers protocol

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Lyophilized (freeze-dried) powder: Room temperature storage: stable for weeks to months depending on peptide Refrigerator storage (2-8 C): stable for months to over a year Freezer storage (-20 C): stable for years in some cases Keep sealed, dry, and away from light Moisture is the primary enemy of lyophilized peptides Reconstituted solution: Must be refrigerated at 2-8 C at all times Typical stability: 4-6 weeks for most peptides Never freeze reconstituted solutions Use bacteriostatic water (not sterile water) for multi-use vials Each needle puncture introduces contamination risk The difference in stability is dramatic