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Trypsinised peptides were collected from gel pieces via centrifugation, followed by further extraction 30 min incubation at RT with 99.8% (v/v) ACN/0.2% (v/v) formic acid and then 50% (v/v) ACN/0.1% formic acid (v/v) followed by centrifugation

Instead of dissolving l-ascorbic acid in the base during production, the active powder remains isolated in a separate internal chamber

They also said vitamin B12 was given as an adjuvant drug with patients current treatment regimen, including fibromyalgia drugs, and that may have changed the effect of vitamin B12

In conclusion, pre-clinical data of TLR3 agonists provide promising perspectives for them to move from bench to bedside, yet more research and trials are needed in optimizing TLR3Ls clinical effectiveness

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