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Staining incubation times lasted four minutes for all primary antibodies and two minutes for secondary antibodies

NF-B, Nuclear Factor Kappa B

Nutrition and Metabolism 2010;7(30):1-14

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To make incubation plates 500 l heat-inactivated OP50 (65 C, 30 min) was mixed with 20 l MitoTracker Red CM-H 2 X stock solution (0.5 mM) and 50 l spotted on a NGM agar plates